Visual detection method

Method * Virus Detection Reference
Subfamily Genus Species ** Virus Target gene/region *** Host Sample Limit

* LAMP: Loop-mediated isothermal amplification; RPA: Recombinase polymerase amplification; RAA: Recombinant enzyme-assisted amplification; CPA: Cross-priming amplification; UCPA: Uracil-DNA glycosylase (UDG)-supplemented cross-priming amplification; GICA: Colloidal gold immunochromatographic assay; LFD: Lateral flow dipstick; LFS: Lateral flow strip; LFA: Lateral flow assay; LFB: Lateral flow biosensor; VF: Vertical flow visualization; SPM: Smartphone microscopy; XO: Xylenol orange; HNB: Hydroxynaphthol blue.

** LCDV: Lymphocystis disease virus; ISKNV: Infectious spleen and kidney necrosis virus; SDDV: Scale drop disease virus; CMTV: Common midwife toad virus; FV3: Frog virus 3; SCRAV: Santee-Cooper ranavirus; SGIV: Singapore grouper iridovirus; DIV1: Decapod iridescent virus 1.

*** MCP: Major capsid protein gene; ATPase: Adenosinetriphosphatase gene; DPOL: DNA polymerase gene; RAD2: DNA repair protein gene; TK: Thymidine kinase gene.

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